Application of Repetitive Extragenic Palindromic sequence (REP) as molecular marker for biotyping Aeromonas strains isolated from surface and sub- surface soil sediments
Aeromonas species are ubiquitous bacteria in terrestrial and aquatic milieus. They are becoming renowned as enteric pathogens of serious public health concern as they acquire a number of virulence determinants that are linked with human diseases, such as gastroenteritis, septicaemia, and skin diseases. Identification of Aeromonads to the species level is problematic and complex due to their phenotypic and genotypic heterogeneity. With the aim of clarifying taxonomic relationships among the strains, Repetitive Extragenic Palindromic sequence (REP) was used as molecular marker for biotyping Aeromonas strains isolated from surface and sub- surface soil sediments from Forest ecosystem. Out of a collection of 120 strains isolated from surface and sub-surface soil sediments, 15 strains were confirmed as Aeromonas based on phenotypic fingerprinting obtained from 69 biochemical tests. Genetic diversity among Aeromonas sp. was determined by analysing their REP fingerprint patterns using NTSYS pc software package and UPGMA cluster method. Considerable amount of genetic diversity was observed among the isolated strains. Furthermore, dendrogram demonstrated the existence of distinctive clusters at various similarity levels. These fingerprinting techniques can therefore be used to recognize the relatedness of strains derived from clinical and environmental isolates, which helps in further characterization of factors/genes responsible for the disease.
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Power quality improvement using three phase harmonic filter and PLL technique
In this proposed work a new technique has been developed for reducing total harmonic distortion using THREE PHASE HARMONIC FILTER and PHASE LOOK LOOP (PLL) technique in HVDC system .This work model implemented in simulation work. Harmonic filter are shunt elements that are used in power systems for decreasing voltage distortion and for power factor correction .Nonlinear elements such as power electronic converters generate harmonic currents or harmonics voltages which are injected into power system. The resulting distorted currents flowing through system impedance produce harmonic voltage distortion. Harmonic filters reduce distortion by diverting harmonic currents in low impedance paths. Harmonic filters are designed to be capacitive at fundamental frequency, so that they are also used for producing reactive power required by converters and for power factor correction. Phase Lock Loop technique is used for harmonic reduction of the system and improving power quality of the hole system. Phase Lock Loop is synthesize the new frequency. In this system reactive power compensation is doing by the two types of compensation devices are shunt compensation and series compensation.
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Study of Protonic Conduction in Aloe-Vera Pulp Based Green Electrolyte System
The polymeric membrane using naturally occurring aloe-vera pulp as host and for filler, fine powdered natural waste egg shell was used which served the purpose of second component of the composite. The ratio of the aloe pulp and egg shell (ES) was kept 90:10. The membrane was characterized by different spectroscopic methods, viz., X-ray diffraction, Fourier transformed infra-red spectroscopy and conductivity by impedance spectroscopy. There is a sharp increase in the resistivity with temperature around melting temperature of the crystalline polymer. The attraction of this work is that the ingredients used for constructing the polymer matrix are natural , biocompatible and fully biodegradable.
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Ethico-Legal Investigation of Patenting of Biotechnological Invention Involving Human Stem Cell
Developments in the life sciences, whether in health, agriculture or the environment, have had an unexpected impact on life expectancy and the quality of life. Further, biotechnological inventions has already provided life-saving medicines such as monoclonal antibodies to treat cancer, human insulin to treat diabetes, erythropoietin to treat anaemia and cling to promise for cures for deceases currently regarded as untreatable. In the past decades biotechnology has been one of the fastest growing fields of technology among all. As the field covers a wide range of areas from micro-organisms to agriculture and medical applications, and involves publicly disputed techniques and products such as genetically modified plants, animal cloning or human embryonic stem cells, the ethical issue on patents is more heated & controversial than in other technological areas.
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A Survey of Attacks in Optimized Link State Routing Protocol
A survey on mobile adhoc network networks have experienced strong growth due to their ability to provide an additional and complementary support for existing infrastructure communication systems. In such a network, routers are supposed to be fixed for short (e.g. public safety deployment) or long (e.g. network operator extension) period. This relative stability of infrastructure makes proactive routing protocols appropriate. One of the well known proactive routing protocols is OLSR (Optimized Link State Routing), which routing decisions are based on exchanges of topology information using all-to-all flooding of local information in order for each router to build a global knowledge of the topology. This study first goal is to improve the performance of topology information flooding in OLSR by introducing network coding techniques, which leads to a decrease of signaling overhead.
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Molecular structure and vibrational spectroscopic analysis of an anti HIV drug Epivir: A combined experimental and quantum chemical approach
Epivir is an anti HIV agent belonging to the class of nucleosides reverse transcriptase inhibitors. These drugs interrupt the virus to make copy of it. A systematic quantum chemical study and vibrational spectra of Epivir has been reported. Structure and spectral characteristic of Epivir have been studied using vibrational spectroscopy and quantum chemical methods. Density function theory calculations of optimized geometry and vibrational spectra have been carried out by Gaussian03, using 6-311G basis set and B3PW91 functional. Based on these results we have discussed the correlation between these vibrational modes and crystalline structure of Epivir. A complete analysis of experimental IR and Raman spectra has been reported on the basis of wave number of the vibrational bands. The IR and the Raman spectra of the molecule based on DFT calculations shows reasonable agreement with the experimental results. The calculated HOMO, LUMO shows that the charge transfer takes place within the molecule.
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Ethical behavior of implanting electronic chip in the brain
We have long used mechanical devices to compensate or physical disability, it may be possible to augmented mental capacity to add memory or carbon-based neural net 'wetware' brains-at a point in history, when we might be able to imprint the circuitry of the human brain using transistors on a silicon chip? Brain Chip-a direct interface between the biological information processing system of the brain and neurons system and the artificial information processing system of microprocessors and silicon electronics. The brain is a parallel processor. The colorful blue jay, I see flitting from tree to tree in my garden appears as a single image. But the brain divides what it sees into four component-color, motion, shape & depth. These are individually processed-at the same time-and compared to my stored memories. My brain combines all of these processes into one image, that's just vision aspect of a multiplexed moment of perception..- The human cortex has about 22 billion neurons and 220 trillion synapses. In this paper, we purpose to initiate ethics of implanting computer chip in the Brain and focuses on issues of manufacturing and scientific responsibility, anxieties about possible usage in children, and most troubling, issues of privacy and autonomy.
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Blockchain for Real Estate Industry
Blockchain technology is one of the latest technologies on the horizon and has evolved over the last 7-10 years. There is tremendous potential for usage of Blockchain technology in today’s Real Estate industry. This paper aims to analyze the disruptive power of Blockchain digital technologies in real estate industry. This paper will start with an introduction of the blockchain technology and high-level technical overview. It will then go on to discuss the various benefits the Real Estate industry can reap from this technology. It will also put forth various use cases or opportunities in the Real Estate sector for this technology. In the end, the paper will also discuss the present-day limitations and the questions marks over this technology in relation to its usage for the Real Estate industry.
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Reliability and sensitivity analysis of a two unit warm standby system with low efficiency unit
In the present paper the system considered consists of two subsystems A and B. Subsystem A consists of identical operating and warm standby units. While subsystem B has two dissimilar units: main unit and a unit in cold standby. Main unit of subsystem B is assumed to be more efficient than the standby unit so when the main unit fails the system goes to the state of low efficiency. Main unit is connected to cold standby unit with a switching over device. Further, whenever there is a failure in one of the units of A and in the main unit of subsystem B, the system goes to critical state where system has to stop functioning to avoid the further failures. Also we have considered that the company providing repair facility has appointed a repairman. The repairman repairs the system in case of minor failures but when the system fails completely he has to take it to the nearest service station of the company for repair. By applying Supplementary variable technique, Laplace transformations and copula methodology transition state probabilities, asymptotic behaviour, reliability, availability, M.T.T.F., cost effectiveness and sensitivity of the system have been determined. Particular cases corresponding to the situations when the standby unit of subsystem A is in cold standby and in hot standby have also been considered. At last some numerical examples have been taken to illustrate the model.
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Vibrational Properties of an Anti-HIV Drug Abacavir: Experiment and Theory
Abacavir (ABC) {(1S,4R)-4-[2-amino-6-(cyclopropylamino)-9H-purin-9-yl] cyclopent-2-en-1-yl} methanol, is an antiretroviral medication which is used to prevent and treat HIV/AIDS. It belongs from nucleoside analog reverse transcriptase inhibitor (NRTI) a sub class of RTIs, which interrupt the virus to make copy of it. In this paper we reported the results of a systematic experimental IR and Raman study of Avacavir. Additionally the equilibrium geometry, harmonic vibrational frequency Raman and IR intensities were calculated using density function theory. Finally complete vibrational assignment of Abacavir is given for the observed Raman and Infrared spectra. The experimental and theoretical results are found to be in excellent agreement with each other. We believe that the results obtained herein will prove to be an excellent starting point for studying the detailed potential surface of the molecule which is needed to understand the drug receptor interactions.
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