Biosciences

Piroxicam, an anti-inflammatory drug, exhibits poor water solubility, dissolution and flow properties. Thus, the aim of the present study was to improve the solubility and dissolution rate of Piroxicam by preparing microspheres by spray drying technique. Piroxicam microspheres containing different ratio of F127 were produced by spray drying using dichloromethane as solvent to enhance solubility and dissolution rate. The prepared formulations containing different ratio of drug and polymer were evaluated for in vitro dissolution and solubility. The prepared formulations were characterized by scanning electron microscopy, differential scanning calorimeter, X-ray diffraction and Fourier transform infrared spectroscopy. Dissolution profile of the spray dried microspheres was compared with its physical mixture and pure sample. Spray dried microspheres exhibited decreased crystallinity and the solubility and dissolution of the microspheres containing different ratio of drug and pluronic F127 were significant improved compared with its physical mixture and pure sample of piroxicam. Dissolution of microspheres containing 1:3 w/w (SD 3) showed higher % release i.e. 94 % in 60 min compare to other formulation. Consequently, Hence, From the above result it can be conclude that spray dried microspheres of Piroxicam is a useful technique to improve the solubility and dissolution of poorly water soluble drug like Piroxicam.

Biosynthesis of silver nanoparticles using Diospyros chloroxylon Roxb. ripened fruit pulp of extract was investigated and the effect of broth concentration in reduction mechanism and particle size is reported. The rapid reduction of silver (Ag+) ions was monitored using UV-visible spectrophotometer and showed formation of silver nanoparticles within 20 minutes. Transmission electron microscopy (TEM) showed that the synthesized silver nanoparticles are varied from 10-25 nm and have the varying in shapes like spherical, round uneven. Further the XRD analysis confirms the nano-crystalline phase of silver with FCC crystal structure. FTIR examinations confirms the Silver particles. The present study, it was found that the increasing broth concentration increases the rate of reduction and decreases the particle size.

This study was designed to investigate the biochemical effects of graded doses of aluminum on some serum lipid profile (SLP) of male wistar albino rats. A total of twenty-four male albino rats of 10-12 weeks of age were used for the study. They were randomly assigned to six groups (Groups A-D) of six rats each. The treatment groups-A to C were administered aluminum as aluminum chloride (AlCl3) : 0.38, 3.8, and 38 mg/kg body weight while group D received 0.2ml normal saline which served as vehicle. Assay of the SLP were carried out using standard biochemical methods after 14 days. The results showed that serum total cholesterol of the treatment group administered 38mg/kg decreased significantly (p<0.05) relative to the control whereas the serum low density lipoprotein (LDL) of the treatment groups administered 3.8mg/kg and 38mg/kg decreased significantly (p<0.05) relative to the control after 14 days of treatment. The treatment group administered 0.38mg/kg showed a decrease in serum total cholesterol and low density lipoprotein but were not statistically significant (p>0.05).

Breath Management is currently a very important topic in the singing community. Taking into consideration the earlier studies, singing requires a higher rate of breath energy than speaking does, as well as the elongation of the breath cycle and breath holding capacity. Breath holding capacity was measured using Sabraze’s breath hold test. Methods: 94 subjects, In group A: 47 subjects and in group B: 47 subjects were recruited in this study by convenient sampling. Measuring tool: Stopwatch. Results: Our results reveal better breath holding capacity amongst Indian classical singers than contemporary singers (p<0.05). CONCLUSION: Indian classical singers were found to have better breath holding capacity than contemporary singers

In this work two samples of a (Pandanus amaryalifolious) leaves extracts, were exposed to sound waves length of (528 nm and 741 nm) while the third sample of the leaves was left without exposure to any sound. There was difference of absorption in each sample; these differences were detected using X-Ray Tubes Techniques. The difference of absorption showed the range of germination is not the same in the three samples. As well as the difference in the combination’s bonds of the fundamental molecular vibrations as well.

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major causes of nosocomial infections worldwide and is responsible for significant morbidity and mortality of the patients. It is usually resistant to many antibiotics and therefore diseases caused by MRSA are more difficult and expensive to treat than methicillin-sensitive Staphylococcus aureus (MSSA). The aim of this present study was to determine the prevalence of MRSA infections and their antimicrobial susceptibility pattern in our hospital located in Central Nepal. Clinical specimens received from October 2016 to March 2017 in Bacteriology Laboratory of Microbiology, College of Medical Sciences-Teaching Hospital. The specimens were processed and identified by standard laboratory procedures. The antibiotic susceptibility testing of all included staphylococcal strains was performed by modified Kirby Bauer disc diffusion method. During this study period, a total of 173 isolates of S. aureus were studied and 75 (43.35%) were found to be Methicillin-resistant S. aureus (MRSA). In our study maximum percentage of S. aureus (49.13%) and MRSA isolation (49.33%) were from pus and wound swab. The majority of the samples were obtained from surgery (53.19%) and ICU (19.15%). In the study penicillin, oxacillin resistance was observed to be 100%, 43.35% respectively against the organism. About 50–55% of MRSA were resistant to erythromycin, gentamicin and ciprofloxacillin while less than 30% were resistant to levofloxacillin. However, this present study also indicates the emergence of Vancomycin Resistant S. aureus (VRSA)-9.25%. To reduce the prevalence of MRSA and its spread to community as well, the regular surveillance of hospital-associated infections, isolation of patients who carry MRSA, monitoring of antimicrobial susceptibility pattern and formulation of a definite antibiotic policy may be helpful.

The apparent and thermodynamic parameters (pKa, pKb, Ka, Kb) are among the most common used physical parameters and their determinations are of interest to a broad range of research. It is apparent that hypersaline hydrochemical environment in concentrated salt lake brines has a profound modifying influence on parameters such as Ka, pKa's and buffer capacities. This study presents chemical parameters (Ka, pKa and buffer capacities) at typical high ionic strength salt lake brines, which were evaluated at 25 oC and have revealed both pKa1 and pKa2 to decrease as ionic strength of the solution increases. The apparent dissociation constant Ka for HF is increasing as the ionic strength increases and thermodynamic Ka = 7.46 x 10-4 found is in good agreement with the values reported in the literature. The phosphate buffer capacity in the target Rift Valley Salt Lake systems is observed to exceed that of pure water by a factor of 36 to 1000.

The aim of the present work was to develop a Itraconazole freeze dried tablet (FDT). Which dissolve instantaneously in the mouth. To enhancing the bioavailability of poorly water-soluble Itraconazole was improved by preparing a FDT of Itraconazole using freeze-drying technique. The FDT was prepared by dispersing the drug in an aqueous solution of highly water-soluble carrier materials consisting of gelatin, glycine, and sorbitol. The mixture was poured in to the pockets of blister packs and then was subjected to freezing and lyophilization. The FDT was characterized by DSC, XRD and SEM and was evaluated for saturation solubility and dissolution and compared with physical mixture (PM) and pure drug. The samples were stored in stability chamber to investigate their physical stability for 90 days. Result obtained by DSC and X-ray studied showed that Crystalline state of Itraconazole in FDT transformation to amorphous state during the formation of FDT. SEM result suggests reduction in Itraconazole particle size. The solubility of Itraconazole from the FDT showed seven times greater than pure drug was due to super-saturation generated by amorphous form of the drug. Dissolution studies showed that dissolution rate of FDT of Itraconazole significantly improved compared with the PM and the pure drug. More than 90% of Itraconazole in FDT dissolved within 5 min compared to only 27.38% of Itraconazole pure drug dissolved during 60 min. In stability test, the dissolution release profile of the Itraconazole in FDT was almost unchanged as compared with the freshly prepared FDT.

Mithi River is one of the polluted rivers in Mumbai. It is most vulnerable to pollution from discharge of sewage through municipal outlets as well as improper outlet channels connected through slums and further by industrial effluent discharges containing heavy metals. Isolation and narrowing down of copper resistant bacteria were carried out from samples collected from different locations on the river. It was screened for resistance at different concentrations of Copper, i.e. 50ppm, 100ppm, 500 ppm and 1000ppm. The resistant bacteria revealed that it belonged to the staphylococcus genus. Bioremediation studies were carried for a period of 120 hours in the medium. The decrease in metal was analysed at 24 hours and 120 hours. The approximate removal capacity of copper in the medium was observed to be 32% and 84% respectively.

Cholesterol is a component of cell membrane and a precursor for steroid hormones and bile acids synthesized by the body cells and absorbed with food. Cholesterol has been identified as one of the major factors that affect and sometimes destroy the normal functions of the heart blood vessel. This study determines the cholesterol level of some selected bank workers of Ghana Commercial Bank (GCB) at Accra New Town Municipality between the ages of thirty five – forty five years respectively. Out of the twenty five (25) samples assayed four males were found above the normal cholesterol range of 3.5 - 5.5 mmol/l, and six females were found above the normal cholesterol range. In all 10 were found above the normal range while 15 were found within the normal range (3.5 - 5.5 mmol/l). The result shows that 40% of the women had high level of cholesterol while 60% of the men had a high level of cholesterol. In all 60% of the bank workers had high cholesterol level as compared to the reference range while 40% of the bank workers had their cholesterol levels within the reference range. About 26.66% were found to be females who were obese whilst 40% were also obese males.