Activity of an enzyme ATPase in an alga Anabaena cylindrica, Lemm. with a Pesticide Sevin Under Laboratory Condition
Pesticides or agro-chemicals play a pivot in controlling pests on agricultural and horticultural crops. SEVIN 50% W.D.P. based on carbaryl. 1. Naphthylmethyl carbamate, is a broad spectrum pesticide for control of pests on fruits, vegetables, forage, cotton and other crops, as well as poultry and pets. In order to know the extent of toxicity five different concentrations of the toxicant ( Sevin) are taken – LC0 (2.13 ml. l-1)) , LC10 (2.54 ml. l-1) ), LC50 (3.01 ml. l-1) , LC90 (3.25ml. l-1) , LC100 (3.35 ml. l-1) . Uni -algal, axenic culture of Anabaena cylindrica, Lemm. was inoculated and the survival percentage was determined. The ( LC10 ,LC50 , LC90) three lethal concentrations were chosen to study the differential effects of different concentrations of the pesticide (Sevin) on the blue-green alga, Anabaena cylindrica, Lemm. The changes in the ATPase activity (?g ip liberated hr -1 50 ml culture) of the alga Anabaena cylindrica, exposed to different concentrations of the pesticide, Sevin, at different days of exposure and recovery. The enzyme activity values were far less than the control value and even less than the inoculation day value, except the 3rd day of exposure. No significant recovery was marked, indicating total destruction of the enzyme system in the exposed alga. The percent change in ATPase activity showed non-significant negative correlation with the exposure period in conc. A and B. However, in conc. C, a negative significant (r= -0.974; p < 0.01) correlation was marked. The ATPase activity increased in Conc. A (2.5 ml l -1) up to 12th day of exposure, when compared to the control value and then the enzyme activity decreased. With the increase in pesticide concentration and exposure period, the enzyme activity significantly declined, when compared to the control value. A maximum of 93.5% and 99.1% decrease was recorded on 15th day of exposure and 15th day of recovery in conc. C.
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Adjusting to the University and College Way of Life among the First Year Diploma Students in Uasin-Gishu County, Eldoret Town Municipality, Kenya
The transition of the students from secondary school to college/university level becomes a major challenge for majority of the students in Kenya. Moreover, compounding challenges have increasingly affected the student population towards adjusting in the university level. It is therefore recommended that a strategic orientation programme especially on guidance and counselling services for the first year diploma students. Accordingly, this paper advocates for the review of student support programmes towards the adjustment of first year students in the university/college environment. Finally, there is need for the inclusion of psychologists, counselling psychologists and psychiatrists in student support services.
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Analysis of security mechanisms against Denial-of-Service attack in wireless sensor networks
Wireless Sensor Networks are used in numerous security sensitive applications, it composed of a huge number of resource limited sensors therefore we should make sure the network is secured against security attacks. Denial of service attack in WSNs is a serious attack that reduces the network capacity dramatically and the users fail to receive expected service. Many schemes have been proposed to tolerate or destroy DoS attack, but very few can identify intruders and provide security against this attack. In this paper, we explore the effects of DoS in WSN, effective security methods for achieving the security against this attack. Finally two important types of DoS attacks called jamming and reply attack are studied and possible solutions are recommended.
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Analysis on grammatical metaphor from the view of Meta function
Grammatical metaphor is a kind of variation to semantics in different degrees caused by some deviation of grammatical character. From the view of meta function in systematic-functional linguistics, the author discusses three forms of grammatical metaphor systematically: ideational metaphor, interpersonal metaphor, textual metaphor and their techniques of expression.
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Anti-Universe exists
Universe is continuously expanding, and is observed to expand at a continuous increasing speed, much greater than that of light, though the theory of relativity destroys the relevance of this statement. This gave birth to a theory called Big Bang theory, which says universe was born from a single point, started from pure energy and then came matter, space, and time. Within a short time, it is said to have “fight” with anti-matter and a small part of total matter constructed this whole universe.
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Antileishmanial activity of Mangifera indica leaf extracts on the in vitro growth of Leishmania donovani promastigotes
Mangifera indica L. (Anacardiaceae), commonly known as Mango, is a large evergreen tree indigenous to Asia and found throughout the Indian subcontinent. In the present study, the in vitro antileishmanial activity of petroleum ether, chloroform and methanol extracts from M. indica leaf was evaluated against Leishmania donovani (strain AG 83) promastigotes by in vitro promastigote cell toxicity assay by using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide]. Here, all the test extracts markedly inhibited the growth of L. donovani promastigotes in vitro in a dose dependent manner. The methanol extract was found to be the most active followed by the chloroform and petroleum ether extracts respectively. Therefore, from the present study it can be inferred that M. indica leaf extracts exhibited remarkable antileishmanial activity against Leishmania donovani promastigotes in vitro.
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Application of Value Stream Mapping with Value Engineering in Furniture Manufacturing Small Scale Industry
The purpose of this paper is to develop a plan for reducing a lead time in a small scale furniture manufacturing industry by using Value Stream Mapping Technique with Value Engineering. In this paper VSM technique is applied in furniture manufacturing small scale industry to identify the ways of reducing waste while at the same time increasing the proportion of the processes that add value to the product. The wastes in this furniture industry is mostly in the form of over production, transportation and delay. The reasons observed are inadequate coordination among the operators, improper planning, non effective use of machine, improper flow etc. Here the flow process chart is constructed to calculate the number of operation, transportation, delay, inspection and storage with distance travelled by the product for existing and proposed methods in which considerable saving is achieved. Also the Current and Future VSM is created for small scale furniture industry which manufactures office tables. In this the lead time can be reduced considerably with the reduction in NVA time and the process time. There is a considerable rise in VA time of 10.41% with the value addition by modification of the table with less process time.
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Assessment of a Rapid Diagnostic Tool for Sickle Cell Hemoglobin S
Sickle cell disease an inherited common blood disorder due to the presence of the abnormal haemoglobin S. This is a prospective study done at Kenyatta National Hospital that describes a point of care method for diagnosis of HbAS, HbSS, HbSC and HbAA using the Sickle SCAN™ test. It determines the sensitivity, specificity in comparison with haemoglobin electrophoreisis.These results validate the use of sickle Scan as a low cost method for screening and diagnosing children and adults with esickle cell disease.
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Assessment of selected heavy metal residues in meat of cattle in Ondo state, Nigeria
The presence of heavy metal residues in food products is a potential hazard to humans and animals as these metals cannot be degraded and stay permanently in the environment hence there is a need to ascertain the safety of these products for human consumption. The concentration of heavy metal residues in meat, milk and cheese were assessed in three locations in Ondo State. A total number of 120 animals (comprising 30 cows and 90 bulls) and 30 cheese samples were sampled for the investigation. The samples were digested with concentrated nitric acid and the resulting solutions were analyzed using Atomic Absorption Spectrophotometer (AAS) for lead, copper, zinc, cadmium and chromium. The result revealed that the concentration of each metal was significantly (P < 0.05) different due to the location. The results were compared with Maximum Recommended Limit (MRL) as set by FAO. The cadmium concentration in the kidney samples obtained from Owo has a mean concentration level of 1.23mg/kg, which is above those collected from Ondo (0.53mg/kg). However, Cadmium was not detected in kidney samples from Akure. Chromium concentration was high in kidney samples from Ondo and Owo with values of 1.11 and 1.21mg/kg respectively than those recorded in Akure (0.31mg/kg). The mean level of copper in skeletal muscle from Akure (3.17kg/kg) was higher when compared with the 2 other locations (Ondo 1.48mg/kg; Owo 1.82mg/kg). The mean concentration of lead in skeletal muscle (2.09mg/kg) and kidney samples (0.89mg/kg) were significantly higher than liver samples (0.78mg/kg). Of these skeletal muscle, kidney and liver samples, 100%, 46.7%, 73.3% exceeded the MRL by FAO respectively. For cadmium, the result showed that kidney samples of slaughtered cattle contained a mean value of 0.59mg/kg. Also, the concentration of zinc in skeletal muscle and liver of slaughtered cattle were 37.65and 50.55mg/kg out of which 36.7% and 43.3% exceeded the MRL by FAO for skeletal muscle and liver respectively. The mean concentration of chromium is higher in kidney samples (0.88mg/kg) than skeletal muscle (0.77mg/kg and liver (0.83mg/kg). Of these skeletal muscle, kidney and liver samples, 16.7%, 36.7%, 20% exceeded the MRL as set by FAO respectively. Copper mean concentration was higher in liver samples (20.57mg/kg) of which 100% of liver samples exceeded MRL by FAO.
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Calibration of harshaw 4500 TLD reader and workplace monitoring of some selected diagnostic radiology centers in ibadan, Nigeria
Calibration of Harshaw 4500 Thermo luminescence Dosimeter (TLD) Reader was carried out using standard X-ray beam. The response of the TLD reader to the increasing dose was found to be linear and the dose response characteristics measured were 0.68 mSv, 1.80 mSv and 3.39 mSv which were comparable respectively to 0.55 mSv, 1.87 mSv and 3.76 mSv obtained following manufacturers specification. However, at doses below 500 mSv deviation was observed between the two procedures. The supervised and controlled areas at the radiology diagnostic centers were monitored using TLD badges over a period of 60 days. The dose levels obtained ranges from 0.13 mSv/hr to 0.2 mSv/hr for the conventional x-ray rooms and 0.10 to 0.35 to 0.16 mSv/hr for the waiting areas. The readings obtained established that the diagnostics centers were free from under exposure to ionizing radiation.
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