Biosciences

The new shielding using a mixed of Barium Sulfate (BaSO4) and cement accordingly to the ratio that desired which is from (1:99) to (20:80) respectively. The samples were in thickness of 1 mm, 5 mm and 10 mm with the ratio of BaSO4 are 0, 5, 10, 15, and 20%.The samples are examined with XRD. The mixture ratio of barium sulfate that has 20% in the sample shows that the absorption of the energy is high. A high mass density material of sample as barium, which has high atomic number is suitable to absorb the energy of radiation. Increasing the material density leads to increase the attenuation. The results have shown a significant protection for hazardous of gamma ray.

Proteus mirabilis is a significant problem mostly to the vulnerable immune systems individual and cause of 90% Complex Urinary Tract Infections. The treatment is becoming more difficult because 48% P.mirabilis strain is resistant to broad-range antibiotics. Development of these drug resistant varieties have led to search for novel drug targets. We have performed an insilico comparative analysis of metabolic pathways of Homo sapiens with the pathogen P.mirabilis and found 97 unique, nonhomologus, essential proteins that are present only in P.mirabilis and absent in humans and presented as list of putative drug target.

Enzymatic potentials of thermo-tolerant bacteria from waste dumpsites were analyzed. 30 soil samples were collected using sterile spatular into sterile universal bottles, labelled and taken to the laboratory analyses. The soil samples were serially diluted and inoculated by spread plate method on different media and incubated at 30oC for 48 hrs. The pure isolates were stored in agar slants in the refrigerator for further use. Isolates with thermophilic ability were screened for amylase, protease and lipase activity. Effects of temperature and pH on enzyme activity of the thermophilic bacterial isolates were also assessed. Bacillus subtilis (70.0%), Bacillus licheniformis (43.3%), Bacillus cereus (56.7%), Pseudomonas aeruginosa (56.7%), Streptomyces species (36.7%), Bacillus brevis (43.3%) and Norcadia species (30%) were isolated from the soil samples. All the bacterial isolates tolerated 55oC temperature while only Bacillus cereus, Bacillus brevis and Norcadia species were isolated at 60oC. All the isolates except Pseudomonas aeruginosa gave amylase activity while all the isolates gave protease activity. All the isolates except Bacillus licheniformis and Nocardia species showed lipase activity. Temperature and pH had varied effects on the enzyme activity of the isolates. These bacteria are good sources of enzymes at thermophilic temperatures and varied pH for commercial use.

We have been demonstrating that the Distribution of Amino acids in Genetic Disorders. we retrieved that the diseased Proteome sequence for Genetic Disorders such as Anglemann Syndrome, Canavan disease, Celiac disease, Charcot Marie tooth Disease, Colour Blindness, Cridu chat, Cystic fibrosis, Down syndrome, Hemophilia and Neurofibrometasis. The Content of Polar amino acids are higher when compared with other type of amino acids. From the normal sequence, the Amino acids are mutated to Aspartic acid and Glutamic acid. If the amino acids are mutated to hydrophobic, hydrophilic and non polar amino acids we can control the genetic diseases. According to our work, based on Lipinski’s rule we observed that Alverine, Ropinirole ,Cyrimine , Carbidopa and Phosphatidylserine are the best controlling agent for Anglemann Syndrome, Canavan disease, Celiac disease,Charcot Marie tooth Disease, Colour Blindness respectively. We also observed that Mematine,Tyloxapol, Heaprin, Tranexamic acid and Indomethacin are the best drugs for Cridu chat, Cystic fibrosis, Down syndrome, Hemophilia and Neurofibrometasis respectively. Finally we concluded that, if the amino acids are mutated to hydrophobic or non polar amino acids, we can prevent Genetic Disorders.

We have observed that the Immunoglobulin domain (IGv) is mainly present in all those sequences. These observations also suggests that the predicted values of IGv domain is predominantly present in all those sequences and slightly IGc1 domain and IG like domain is also present. On the basis of lower E-value IGv domain is highly conserved in the sequence, from position 17 to 98, and IGc1 domain from 147 to 220.Each sequence has consist of varied composition of CCP (Complementary control proteins), EGF (Epidermal Growth Factor), EGFCA, VWA (Von Wille Brand type A domain), IG, IGc2, TSP1, TROVE, Calireticulin family, RING domain, BBOX, PRY, SPRY with different E-value. Finally, We have concluded that on the basis of lower E-value vWFA(97-214), TROVE(18-369), IGc(183-273), SPRY(86-220), La(13-48), MHC Class I An alpha 1, 2 (1-178) these listed domains are highly conserved.

Fish farming can provide employment for women thus helping to alleviate poverty among women and improve the standard of living. Proceeds from fish sales can provide funds for women and contribute to the development of the society. Fish farmers are known to be confronted with difficulties when establishing commercial fish ponds. This research is intended to cover both Northern and Southern Nigeria. One hundred and thirty questionnaires were distributed randomly in Ilorin, North Central Nigeria to ascertain the constraints to fish farming among women in Nigeria. Ninety questionnaires were retrieved from females. Analysis of results indicated that female respondents were interested in fish farming but they lacked financial capacity to establish fish farms. Education was also identified as another constraint. About 56% of the respondents had basic education therefore were uniformed about fish culture in ponds. Fish farming was identified as a means of providing employment for the women folk and enhancing development among women.

Poly(2-oxazoline)s have widely been used in biomedical applications for the last years. They can mimick natural systems and they generally used as liposomes, drug and gene deliver and pseudopeptides. In this study investigating histological effects of different doses (10 mg/L, 50mg/L) of poly(2-ethyl-2oxazoline) (PEtOx) on liver tissue of zebrafish was aimed. Adult zebrafish individuals were exposed to different doses of PEtOxfor 5 days and after that they were dissected and liver tissues removed. Histological changes at liver tissue were investigated at light microscope after hematoksilen and eosin staining. In the experimental groups, contraction in vacuoles at hepatocyte cytoplasm, hypertrophy and increase in the number of hepatocyte cells and kuppfer cells were detected.

Mancozeb is a synthetic pesticide which has been used for decades. It is used to hinder the growth of fungi and plants against damage. Pesticides access aquatic ecosystems via ground waters and affect water quality and aquatic life. In our study, investigation the histopathological effects of mancozeb on intestine tissue of zebrafish were aimed. Degeneration and expansion at villi structure and increase in the number of goblet cells, mucus accumulation and expansion in muscular layer were detected at exposure groups. Depending on the increased number of leukocytes enteritis was monitored.

This survey achieved in the city of Kindu and his/her/its peripheries have for objective to contribute to the knowledge of the Non Woody Forest products of plant origin, exploited in the biotopes surrounding the city of Kindu, the Nyoka locality, the Bilundu village and the Muyengo village in order to bring the actors to an exploitation responsible for these very important natural resources for their survival. In a specific manner it is about identifying the Non Woody Forest products exploited in the forests of Kindu and his/her/its vicinity and determined their biotopes of exploitation. For what is the collection of the data on the land, the used methodology had been based on the approach ethnoécologique, while leading an investigation on the Non Woody Forest products of the forests of Kindu and his/her/its vicinity. The investigation had concerned the exploiting permanent residents of the city of Kindu (Kindu city, Katako village, Shenge village, District Lwama), of the Bilundu village on the Kibombo road, the Nyoka village and the Muyengo village on the Kalima road. We had pulled at least 25 at random% of the households producers and to manage them the questionnaire elaborated to this effect. This collection of data was achieved as organizing some interviews to the course of which us had filled the cards. Therefore, the unit of sampling is the household of operators and consumers to the level of the survey sites and, our sample was of 697 individuals. The results showed that the species the more exploited like wild food plant in the biotopes of the Nyoka locality is Amaranthus viridis (LINNE), either 31%, followed of Colocasia antiquorum, var. esculenta, either 25% or Xanthosoma sagittifolium, 19%. In the biotopes of the Muyengo village, it is the species Amaranthus viridis that is the more harvested (34%), followed of Colocasia antiquorum (19%) and of Xanthosoma sagittifolium (11%). One also found that in the biotopes of the Bilundu village, one harvests the species more Dioscorea prehensilis (30%), followed of the species Amaranthus viridis (15%) and of the species Pteridium aquilinum (12,4%). Among the wild food plants harvested in the different biotopes of the city of Kindu, the species Amaranthus viridis is the more exploited, either 26%, followed of Xanthosoma sagittifolium, 16% and of the species Colocasia antiquorum and Talinum triangularis (11% for every case). In the Nyoka locality, 40% of forest operators harvest the PFNL of plant origin in the forest, 28% find them in fallows and 19% in the fields. In Muyengo, 38% in the forest, 29% in the fallows and 20% in the fields. In the Bilundu village, 37% of forest operators tell to find the Non Woody Forest products of plant origin in the forest, 23% in the savanna and 20% recover them in fallows. And for the city of Kindu, 37% of operators harvest these resources in the forest, 23% in the savannas and 20% in the fallows.

The present study was undertaken to formulate and evaluate a topical emulsion-gel of a non steroidal anti-inflammatory drug, choosing Nimesulide as a model drug. Eight formulations were prepared with different concentrations of carbopol 934p as polymer, varying the concentration of tween 80 and adjusting the pH to 6.5 and 8.0.The concentrations of nimesulide and isopropyl myristate were 1% w/w and 10% w/w, respectively in all the formulations. The prepared formulations were evaluated for various physicochemical parameters. The release studies were carried out by dialysis membrane grade 150 and hairless rabbit skin later compared with marketed product. The formulations were evaluated for anti-inflammatory activity in carrageen an-induced rat paw oedema model. Subjective analysis of formulations in healthy human volunteers for acceptability was carried out. FT-IR spectra confirm that there is no incompatibility between drug and excipients. The formulation with 1% w/w carbopol 934 P, 10% w/w tween 80 and pH 8.0 was found to possess maximum percentage drug diffusion comparable to marketed preparation. Maximum anti-inflammatory activity was market preparation. The formulations were stable for 60 days as no significant change in physicochemical characteristics and drug release properties were observed. The formulation was found to be acceptable among healthy human volunteers and is comparable to marketed formulation. From the present work, it can be concluded that Nimesulide can be formulated into topical emulsion-gel with better drug release properties and improved pharmacological effect.