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To construct the plant expression vector containing synthetic B. thuringiensis-cry1Ac gene for crop plant transformations to resist the insect Helicoverpa armigera. A newly constructed binary vector containing the T - DNA left border, Kanamycin (kan) as marker gene, glucuronidase (uidA) reporter gene and bt-cry Ac 1 gene which transformed to A. tumefaciens by helper plasmid pRK2013, which provides tra and mob genes required to transfer the DNA. The plasmid constructed from basic vector pUC118 containing synthetic Bt-cry 1 Ac gene and pGPTV was restricted digested by EcoRI and Xbal. The digested plasmids were purified, quantified and ligated before triparental mating method of competent cell transformation. The triparental mating efficiency can be observed through back transformation of gene from A. tumefaciens to E.coli. The confirmation of Bt-cry 1 Ac gene in the construct was done by Polymerase Chain Reactions (PCR). Construction of a Bt-cry Ac1 expression vector was successful and this study will be a feasible approach for the genetic improvement of an economically important Crop plants.

In Kenya, the government’s priority in education is in ensuring that quality is maintained at the Directorate of Quality Assurance and Standards (DQAS) which is mandated to coordinate, follow up, and advise on curriculum delivery at school level. The Quality Assurance and Standards Officers (QASO’s) are members of that Directorate and are expected to uphold quality. The study investigated on the influence of QASO’s’ instructional supervisory practices on curriculum implementation in public primary schools in Uasin Gishu County in Kenya. The research study adopted a descriptive survey design. Simple random sampling was used to select 10% of the128 public schools in the County. The study sample was composed of 1 Sub-County Quality Assurance and Standards Officer and 6 zonal QASO’s who were purposively selected from each zone in the Sub-County. From the sampled 56 schools, the 56 head teachers were automatically selected and 168 teachers were randomly selected from those schools. Questionnaires were used to gather data from teachers and head teachers. Data was analyzed using SPSS and findings were presented in frequency tables. The study findings established that QASO’s visited other schools once per year but they carried out their assessments in most of the schools once a term. On coordination of cocurricular activities, QASO’s rarely organized or coordinated co-curricular activities. During assessments, QASO’s advised teachers and head teachers on co-curricular activities. The study recommended that QASO’s supervisory visits should be done twice in a term. QASO’s should accord co-curricular activities equal measure of attention during assessment for promotion of all goals of education and development of an all-round individual in the learner.

Carburizing, though a widely used industrial thermo chemical diffusion process, it is associated with the problem of shape and size distortion in the carburized parts.  These distortions are troublesome as they adversely affect the performance of the parts in terms of life, and trouble free operation. The main objective of our present work is to optimize the distortion level, optimum case depth, and surface hardness value of the carburized parts made of EN 353 material. Taguchi’s mixed level series Design of Experiment was selected for optimization.  The significance of our study was that all the three stages of carburizing (Pre carburizing, Carburizing and Post carburizing) were considered for optimization.  An orthogonal array and ANOVA were employed to investigate the influence of major parameters on the three response variables namely Distortion level, Surface hardness and Case depth and optimum conditions were arrived at by applying high penetration depth, high hardness and low distortion are better as the strategies.

Previous studies emphasizing that psychological interventions and pharmacological medications may have a small effect on depressive symptoms in coronary artery patients, and no beneficial effects on reduction of inflammatory markers and mortality rates. Recently there is increasing interest in whether positive psychological states have predictive power over and above depressive symptoms for cardiovascular disease and all-cause mortality. The present study has intended to test and compare the correlation of plasma high sensitivity C-reactive protein and cortisol awakening response’s flexibility with positive psychological states and depression in 54 coronary artery patients. The results of this study indicated that the correlation between high sensitivity C-reactive protein and psychological states only for life satisfaction and subscale of hope "pathways in regard to goals" are significant. Moreover the correlations of cortisol awakening response’s flexibility with happiness, hope and depression were marginally significant. It is possible that directly building the positive psychological states may counteract negative symptoms and may also buffer against their negative biological outcomes.

This paper presents the effect of Treated Domestic Waste water (TDW) and lime dosage on cube compressive strength of cement mortar prepared with ordinary port land cement and Portland pozzolana cement. The treated domestic waste water was used as replacement to portable water in the proportion of 0,25,50,75 and 100%. From all the replacements, 50% TDW is noticed as effective based on cube compressive strength. This replacement was kept constant and the lime is added to the effective replacement of portable water in the proportion of 0 to 30 with an increment of 5% by weight of water. From the results is found that, for OPC and PPC mortars the optimum dosages were noticed as 20 and 25% respectively. To compute compressive strength results for lime based mixes a regression model was developed and it was verified with experimental results.

In this paper, fatigue damage accumulation were studied using three methods i.e. Miner, new non-linear model and experimental method. The prediction of fatigue lifetimes based on Miner method are uneconomic and non-conservative respectively. However satisfactory predictions were obtained by applying the non-linear damage rule (present model) for 5052-H34 aluminum alloy. Many shortcomings of the Miner methods are related to their inability to take into account the surface treatment effect.

The goal of this paper is to design and implementation of wearable device which can be use as a guide to blind people by allowing them to imaging and response to spatial information by using IR transceiver then give an alarm as a vibration to skin by using a mobile vibrator. This vibration is give alarm to blind person to avoid obstacle, its look like sensory appendages of insects. This device can be used instead of dogs or cane to help blind people also it can be used by workers whose work in dangerous construction locations.

Cholesterol is a component of cell membrane and a precursor for steroid hormones and bile acids synthesized by the body cells and absorbed with food. Cholesterol has been identified as one of the major factors that affect and sometimes destroy the normal functions of the heart blood vessel. This study determines the cholesterol level of some selected bank workers of Ghana Commercial Bank (GCB) at Accra New Town Municipality between the ages of thirty five – forty five years respectively. Out of the twenty five (25) samples assayed four males were found above the normal cholesterol range of 3.5 - 5.5 mmol/l, and six females were found above the normal cholesterol range. In all 10 were found above the normal range while 15 were found within the normal range (3.5 - 5.5 mmol/l). The result shows that 40% of the women had high level of cholesterol while 60% of the men had a high level of cholesterol. In all 60% of the bank workers had high cholesterol level as compared to the reference range while 40% of the bank workers had their cholesterol levels within the reference range. About 26.66% were found to be females who were obese whilst 40% were also obese males.

The high levels of work engagement are when employees are involved with, committed to, enthusiastic, and passionate about their work. This study emphasize that knowledge about engagement and understanding practical issues of engagement in the organisation are equally salient when thinking researching and practicing on the topic employee engagement. Engaged employees can help organization achieve its mission, execute its strategy and generate important business results. For this reason it is important to analyse this study and consider the findings and suggestions composition while paying attention on improving employee engagement decisions for any organisation during the suitable situation.

Dyke-Davidoff-Masson syndrome is a rare entity characterized by hemi cerebral atrophy/ hypoplasia secondary to brain insult in fetal or early childhood period. We present a case of a 10-month-old girl, presenting since 5 months right sided body weakness and generalized tonic-clonic seizures. Brain MRI revealed hemicerebral atrophy of the left hemisphere with dilated ventricles on the same side, cerebral infarction, elevation of the sphenoid wing and petrous temporal bone, and slight calvaria hypertrophy.